Lyo-Ready Non-Bac DNA qPCR Mix
Key Product Features
Description
Lyo-Ready Non-Bac DNA qPCR Mix is a one-tube, animal-origin-free formulation containing optimized buffer chemistry, PCR enhancers, dNTPs (including dUTP), MgCl₂, and Glycerol-Free Non-Bac HS Taq polymerase (HC). The polymerase is recombinantly produced in a eukaryotic system, significantly reducing bacterial DNA contamination and minimizing false positives, particularly critical when targeting prokaryotic genomes. This makes the mix highly suitable for low-abundance targets and multiplex PCR, delivering high sensitivity, specificity and reproducibility.The formulation is glycerol-free and includes specialized excipients to support lyophilization. After freeze-drying, assays can be shipped and stored at ambient temperature, enabling:
- Extended shelf life
- Simplified workflows
- Reduced risk of handling errors
- Greater flexibility in assay deployment
Key points:
- Supports the development of clinical, microbiome, environmental, water and food assays
- Enables highly sensitive multiplex detection of low-copy targets
- Reduces false positives caused by background bacterial DNA
- Flexible format: use as a liquid or lyophilize for ambient-stable assays
| Reagent Name | Volume |
|---|---|
| Lyo-Ready Non Non-Bac DNA qPCR Mix, 4x | 5 μL |
| Primer-Probe Mix, 20x | 1 μL |
| Water | Up to 10 μL |
Figure 1. Lyo-Ready Non-Bac DNA qPCR Mix. Combine reagents as shown for a total reaction volume of 20 μL. Briefly vortex and spin down briefly in a microcentrifuge. Incubate the reaction for 2 minutes at 95°C and then cycle for 5 seconds at 95°C and 20 seconds at 60°C for 45 cycles.
Lyo-Ready Non-Bac DNA qPCR Mix, MDX362
Produced in a eukaryotic system to eliminate E. coli DNA contamination this glycerol-free, bacteria-free, qPCR master mix designed for highly sensitive, low-copy and multiplex qPCR, enabling with reduced false positives.
Documents & Resources
FAQs: Lyo-Ready Non-Bac DNA qPCR Mix
What does “Non-Bac” mean in this qPCR mix?
“Non-Bac” refers to a low bacterial DNA (low bioburden) formulation. The polymerase is produced in a eukaryotic expression system, minimizing residual E. coli DNA contamination that can lead to false positives, particularly when detecting bacterial or low-copy targets.
Why is low bioburden important for qPCR?
Background bacterial DNA can be amplified during qPCR, especially in highly sensitive or multiplex assays. Reducing this contamination improves assay specificity, confidence in low-copy detection and reproducibility across runs
Is this mix suitable for detecting bacterial targets?
Yes. It is specifically designed for applications where bacterial DNA detection is required, as the low bioburden formulation minimizes the risk of false positives from contaminating DNA.
Can this mix be used for multiplex qPCR?
Yes. The optimized buffer chemistry and enzyme system support highly sensitive multiplex assays, enabling reliable detection of multiple targets within a single reaction.
What is the advantage of using a eukaryotic expression system?
Producing the polymerase in a eukaryotic system reduces carryover of bacterial DNA from host cells. This results in lower background contamination, improved assay sensitivity and greater confidence in results involving prokaryotic targets
Is the mix compatible with lyophilization?
Yes. The formulation is lyophilization-ready, containing excipients that preserve enzyme activity during freeze-drying and rehydration.
Can the mix be used in liquid format as well?
Yes. The mix is designed for dual use as a standard liquid qPCR master mix and as a lyophilized reagent for ambient-stable workflow
What are the benefits of lyophilizing this mix?
Lyophilization enables ambient temperature storage and shipping, extended shelf life, reduced cold chain requirements and simplified assay workflows.
Does the mix contain dUTP?
Yes. The inclusion of dUTP supports carryover contamination control systems (e.g., UNG-based workflows), helping to prevent amplicon contamination between runs.
Is UDGase supplied in the mix?
No, UDGase (Uracil DNA Glycosylase) needs to be added separately where there is a greater risk of false positives from cross-over contamination.
Is the formulation animal-origin-free?
Yes. The mix is animal-origin-free, supporting regulatory and quality requirements for diagnostic assay development.
What applications is this mix best suited for?
Typical applications include molecular diagnostics development, environmental and wastewater testing, microbiome research, food safety testing and any assay requiring low background DNA contamination.
Does the mix improve detection of low-copy targets?
Yes. The combination of low bioburden and optimized chemistry improves sensitivity and reduces background noise, making it well suited for low-copy number detection.
Can this mix help reduce false positives?
Yes. By minimizing contaminating bacterial DNA and supporting dUTP/UNG workflows, the mix helps reduce false positives caused by both environmental contamination and PCR carryover.
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