Glycerol-Free Thermostable DNA Helicase (HC)
Glycerol-Free Thermostable DNA Helicase (HC) is a high concentration, glycerol-free mix multi-domain DNA Helicase enzyme, that uses ATP hydrolysis to unwind DNA duplexes with 3’ to 5’ polarity without a requirement for an overhang structure.
Key Product Features
- Unwinds double-stranded DNA
- Operates at up to 65°C, ideal for demanding diagnostic workflows
Description
Glycerol-Free Thermostable DNA Helicase (MDX242) is a highly stable, multi-domain enzyme derived from the Thermoanaerobacter tengcongensis uvrD gene (Tte UvrD helicase). It unwinds double-stranded DNA with 3’ to 5’ polarity using ATP hydrolysis without requiring an overhang structure. Capable of generating single-stranded templates without thermocycling, it remains functional at temperatures up to 65°C.
Key points:
- Glycerol-Free Thermostable DNA Helicase generates single-stranded templates without thermocycling (resistant up to 60-65°C)
- Ideal for robust, efficient, and precise DNA amplification
- Broader applications include NGS library preparation, SNP detection, mutation analysis, and coupling with CRISPR-based systems to enhance DNA accessibility for Cas enzyme binding
Glycerol-Free Thermostable DNA Helicase (HC) was used in a DNA unwinding assay. Glycerol-Free Thermostable DNA Helicase (HC) was used at 1 ng/μL with 50 nM of double-stranded DNA substrate in Lyo-Ready Thermostable Helicase Reaction Buffer, compared to another supplier under the same conditions. The results demonstrate that Meridian’s Glycerol-Free Thermostable DNA Helicase (HC) has a higher reaction rate than other suppliers.
Glycerol-Free Thermostable DNA Helicase (HC), MDX242
Glycerol-free, high concentration enzyme used to unwind double-stranded DNA. It operates at up to 65°C, ideal for demanding diagnostic workflows such as NGS library preparation, SNP detection and mutation analysis
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FAQs: Glycerol-Free Thermostable DNA Helicase (HC)
Do I need to add ATP for Glycerol-Free Thermostable DNA Helicase (HC) activity?
The Lyo-Ready Thermostable DNA Helicase Reaction Buffer, 5x contains ATP.
Does Glycerol-Free Thermostable DNA Helicase (HC) require a particular DNA structure to unwind the strands?
No, Glycerol-Free Thermostable DNA Helicase (HC) can unwind any dsDNA structure.
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